Advance your epigenetics research with NGS methods.
The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.
By the end of this workshop the participants will:
Check our new course layout.
Our trainers have a proven record of academic and/or industrial experience in NGS data analysis. Because up-to-date expert knowledge is needed to answer your questions and know what is important in the field.
We only use open source tools that are free to use. We try to showcase and compare different tools (e.g. NGS mappers), since not every software fits with every task/question.
For a optimal learning experience we carefully prepare our learning materials and example data. All materials, tools and results created during the course are for take home with you.
This workshop has been redesigned and adapted to the needs of beginners in the field of NGS bioinformatics and comprises this three course modules:
Dr. Helene Kretzmer (University Leipzig)
Helene is working on DNA methylation analyses using high-throughput sequencing since 2011. She is responsible for the bioinformatic analysis of MMML-Seq study of the International Cancer Genome Consortium (ICGC). Publications
Dr. Christian Otto (Semless NGS)
Christian is one of the developers of the bisulfite read mapping tool segemehl and is an expert on implementing efficient algorithms for HTS data analyses. Publications
Dr. Mario Fasold (ecSeq Bioinformatics)
Mario works in the analysis of microarray data since 2007 and developed several bioinformatics tools such as the Bioconductor package AffyRNADegradation and the Larpack program package. Since 2011 he specialized in the field of NGS data analysis and helped analysing sequecing data of several large consortium projects. Publications
The target audience is biologists or data analysts with no or little experience in analyzing NGS data. A fundamental understanding of molecular biology (DNA, RNA, gene expression, PCR, ...) is assumed.
A basic knowledge of Linux & Bioinformatics (commandline usage, common commands and tools) is beneficial, but not required. You could prepare yourself with the Learning the Shell Tutorial.
Location: PC-College, Stresemannstraße 78, 10963 Berlin, Germany
Available seats: 25 (first-come, first-served)
Registration Fee: 998 EUR (excluding VAT)
Travel expenses and accommodation are not covered by the registration fee.
Opening Date of Registration: 01 February 2017
Closing Date of Registration: 01 November 2017
Workshop: 29. November - 1 December 2017 (9 am - 5 pm)
"The course was splendid, the instructors did a great job! It generally increased my knowledge on DNA methylation analysis and also taught me some tools/commands I can apply when handling bisulphite data." Ogechukwu Brenda Agba, Leibniz Institute for Age Research, Jena, Germany
"The mix between theory and practical work is very balanced. I highly recommend this course for a strong foundation in the latest methylation analysis methods. The course leaders are experts and so helpful, and to take the operating system, data and commands home at the end of the conference is a huge benefit." Kieran Meade, The Irish Agriculture & Food Development Authority, Carlow, Irland
"Very interesting and useful workshop. Good way to get in contact with other people in the field and have nice discussions. Objectives are clear and well explained and processed." Danny Misiak, Martin-Luther-University Halle-Wittenberg, Germany
When you register for this workshop you are agreeing with our Workshop Terms and Conditions. Please read them before you register.