Support the next-generation sequencing knowledge bank

Welcome to our support page. It provides information for your everyday work with next-generation sequencing data as well as our tools and services. Frequently asked questions by our customers are answered here.


Recent posts

How does bisulfite sequencing (WGBS/RRBS) work?
Convert an interleaved fasta file to a single line fasta using only the Linux command line
How are base qualities calculated and stored?

General NGS analysis

NGS Benchmark - comparison of different read mappers
How are base qualities calculated and stored?
How do strand specific sequencing protocols work?
Are there regions in the genome that are not covered by DNA sequencing?
Is there a bias after DNA fragmentation?
What is mate pair sequencing for?
Do you have two colors or four colors in Illumina?
Why does the per base sequence quality decrease over the read in Illumina?
How to analyze NGS data: An overview of nine different IT solutions
Trimming adapter sequences - is it necessary?
Convert nucleotide sequences with IUPAC codes to an regular expression
What is the best NGS alignment software?

Epigenetics

How does bisulfite sequencing (WGBS/RRBS) work?

Code snippets for sequencing data analysis

Convert an interleaved fasta file to a single line fasta using only the Linux command line
How to get a coverage graph in WIG file format directly from an alignment (BAM)
Convert FASTQ to FASTA on the command line
Annotate mapping entries of a BAM file based on overlaps with BED files using BEDtools
Convert BAM to BED file format using command line perl
Add gzip/gunzip support to a program that doesn't have it (e.g. bowtie)
Extract a list of specific read IDs from a bam file
Run time-consuming processes in parallel on Unix systems