Convert an interleaved fasta file to a single line fasta using only the Linux command line

Most databases provide biological sequences in the multiline fasta format. On the commandline however it is a lot easier to work with a fasta file where the sequence spans just a single line.

The following command snippet allows you to convert you the interleaved format to the single line format using awk, which is installed on most Linux systems by default.

awk '{if(NR==1) {print $0} else {if($0 ~ /^>/) {print "\n"$0} else {printf $0}}}' interleaved.fasta > singleline.fasta


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Last updated on April 26, 2018